Fig. 10

Heat-killed P. intermedia significantly increased the proportions of M2 macrophages and FOXP3 + Treg cells in mouse transplanted tumors. a Representative images of F4/80 (red) and CD206 (green) immunofluorescence staining. White arrows indicate the colocalization of F4/80 and CD206 in the same cell. b Fluorescence intensity difference of CD206 between the control and heat-killed P. intermedia groups. c Fluorescence area difference of CD206 between both groups. d Representative images of CD8α and FOXP3 IHC staining. e The number difference of CD8 + T cells between both groups. f The number difference of FOXP3 + Treg cells between both groups. The data are shown as the mean ± SD and were analyzed by t test. *P < 0.05, ***P < 0.001, ****P < 0.0001. ns, not significant